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14-3-3 Protein epsilon (YWHAE, 14-3-3E)

Cat no: 0014-04G


Supplier: United States Biological
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The 14-3-3 family of proteins mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:3000 Immunohistochemistry (Formalin-fixed, paraffin-embedded): 5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: 0014-04G
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Immunohistochemistry, Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
P type: Mab
Isotype: IgG2a,k
Purity: Purified by Protein G affinity chromatography.
Additional info: Recognizes human YWHAE.

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