14-3-3 zeta is a 245aa containing protein belonging to the 14-3-3 family mediating signal transduction by binding to phosphoserine containing proteins. It is a highly conserved protein in both plants and mammals. The encoded protein interacts with IRS1 implicating a plausible role in regulating insulin sensitivity. There are several transcript variants, with seven known mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta and eta. 14-3-3 proteins function in broad regulation of proteins by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, and activation/repression of enzyme activity. The protein is highly abundant in brain.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.
Positive Control:
Brain
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.