14-3-3 epsilon is a member of the 14-3-3 family which consists of 30kD proteins that are involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 epsilon is expressed in all mammals. It is deleted in small cell lung cancers disrupting the G(2) checkpoint. During apoptosis, cleavage of 14-3-3 protein by caspase-3 increases the affinity between bad and Bcl-x(L). When bound by ERK, 14-3-3 epsilon is thought to deactivate heat shock factor 1 in cells recovering from heat shock. It is also a potential suicide susceptibility gene. May not react with recombinant proteins that are not N-acetylated.
Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:3000
Immunohistochemistry (Formalin fixed paraffin embedded): 1:400
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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