2-phosphoglycerate (2PG) is an important intermediate in the glycolysis pathway. 2-Phosphoglycerate is converted by enolase to phosphoenolpyruvate (PEP) which is a key step from glucose to pyruvate. Aberrant glycolytic metabolism is a highly studied and potentially critical mechanism for ATP generation in cancer cells (The Warburg effect). Measurement of intracellular 2PG levels is a useful tool for analyzing the glycolytic pathway and its relevance to cancer research.
Sample Type:
Animal tissues: e.g. liver, muscle and heart, etc. Cell culture: adherent or suspension cells
Intended Use:
The kit is used for measurement of 2-Phosphoglycerate in various tissues/cells, analysis of carbohydrate metabolism, cell signaling and cancer research.?
Test Principle:
The 2-Phosphoglycerate BioAssay(TM) kit is a sensitive, fast and easy-to-use kit. In this assay, 2PG is converted by Enzyme Mix to PEP, which is further converted to pyruvate. The pyruvate is oxidized to generate color (OD 570nm) and fluorescence (Ex/Em = 535/587nm). The colored product or fluorescence intensity is proportional to 2PG level. This assay kit can detect 2pg level below 20 pmol and can be used for a variety of sample types.
Kit Components:
2PG Assay Buffer, 1x25ml
2PG Probe (in DMSO), 1x0.2ml
2PG Enzyme Mix, lyophilized, 1x vial
2PG Converter, lyophilized, 1x vial
2PG Developer, lyophilized, 1x vial
2PG Standard, lyophilized, 1x vial
Storage and Stability:
Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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