Home  >  Products  >  4E Binding Protein 1 (EIF4EBP1, Eukaryotic Translation Initiation Factor 4E-binding Protein 1, 4E-BP1, eIF4E-binding Protein 1, Phosphorylated Heat-and Acid-stable Protein Regulated By Insulin 1, PHAS-I)
4E Binding Protein 1 (EIF4EBP1, Eukaryotic Translation Initiation Factor 4E-binding Protein 1, 4E-BP1, eIF4E-binding Protein 1, Phosphorylated Heat-and Acid-stable Protein Regulated By Insulin 1, PHAS-I)

4E Binding Protein 1 (EIF4EBP1, Eukaryotic Translation Initiation Factor 4E-binding Protein 1, 4E-BP1, eIF4E-binding Protein 1, Phosphorylated Heat-and Acid-stable Protein Regulated By Insulin 1, PHAS-I)

Cat no: 0004-21N

Supplier: United States Biological
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4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12kD acidic protein that compete with eIF4G for binding of eiF4E to the mRNA 5 cap structure (1). Binding of the 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of 4E-BP. Non-phosphorylated 4E- BP1 will bind strongly to eiF4E while, the phosphorylated form will no (2)t. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 65. Although, not all phosphorylation events equally block the 4EBP1-eIF4E interaction. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot:1:500-1:1,000 Immunohistochemistry:1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: 0004-21N
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
Additional info: Recognizes human 4E-BP1. May cross-react with 4E-BP2 and 4E-BP3. Species Cross-Reactivity: mouse, rat.

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