Home  >  Products  >  4E Binding Protein 1 , phosphorylated (Thr37/46) (Biotin) (4EBP1, 4E-BP1, Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)

4E Binding Protein 1 , phosphorylated (Thr37/46) (Biotin) (4EBP1, 4E-BP1, Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)

Cat no: 0004-19K


Supplier: United States Biological
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Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the eIF4E translation initiation factor. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR on Thr37 and Thr46 does not prevent the binding of 4E-BP1to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5). Applications: Suitable for use in Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight. Flow Cytometry: 1:400. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: 0004-19K
Reactivities: Human, Mouse, Rat, Non-Human Primate
Hosts: Rabbit
Applications: Flow Cytometry, Western Blot
Conjugates: Biotin
Size: 100ul
Form: Supplied in 10 mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
P type: Mab
Isotype: IgG
References: 1. Pause, A. et al. (1994) Nature 371, 762-767, 2. Brunn, G.J. et al. (1997) Science 277, 99-101, 3. Gingras, A.C. et al. (1998) Genes Dev. 12, 502-513, 4. Fadden, P. et al. (1997) J. Biol. Chem. 272, 10240-10247, 5. Gingras, A.C. et al. (1999) Genes Dev. 13, 1422-1437.
Additional info: Recognizes endogenous levels of human 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at analagous sites. Species crossreactivity: mouse, rat, monkey.

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