E-BP1 is a eukaryotic translation initiation factor (eIF4E- binding protein), also known as PHAS-1. The binding of 4E-BP to eIF4E is regulated by phosphorylation and is reversible. Non-phosphorylated 4E-BP1 bind strongly to eIF4E; however, phosphorylated 4E-BP1 will not bind to eIF4E. Akt, TOR, MAP, S6, and Cdc2 kinases are able to inactivate 4E-BP1 by phosphorylation. 4E-BP1 undergoes phosphorylation which releases it from eIF4E, ultimately forming initiation complexes that aid in the upregulation of protein synthesis.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 1:2000 detected 4E-BP1 in NIH/3T3 cell lysate
Optimal dilutions to be determined by the researcher.
Positive Control:
NIH/3T3 cell lysates
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.