Alpha-1 Orosomucoid is a glycoprotein with a molecular weight of 41000. It is an acute phase
protein with a carbohydrate content of 42%. The mean concentration of this protein in serum
is 900 microg/ml. Concentrations are elevated in cases of tissue damage, inflammation,
oestrogen administration and the presence of myeloma.
Applications:
Suitable for use in ELISA, Immunoelectrophoresis and Single and Double Radial Immunodiffusion. Other applications not tested.
Recommended Dilution:
Immunoelectrophoresis: 2ml plasma or equivalent against 120ml antiserum
Double Radial Immunodiffusion: Use a rosette arrangement with 10ml antiserum in 3mm
diameter center well and 2ml plasma samples (neat and serially diluted) in 2mm diameter
peripheral wells.
Single Radial Immunodiffusion: 1% in the gel.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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