Alpha-1 acid glycoprotein (AGP), also known as orosomucoid-1, is an acute phase protein secreted by the liver. Serum AGP levels are elevated during inflammatory responses. AGP binds a wide range of molecules in the circulation. Human and mouse AGP share 43% amino acid sequence identity.
Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Direct ELISA: 0.5-1ug/ml; The detection limit is ~1ng/well
Western Blot: 1-2ug/ml; Detects human AGP in cell or tissue extracts. Buffers used are as follows:
Blotting buffer - 25mM Tris, pH 7.4, 0.15M sodium chloride, 0.05% Tween(R)-20.
Blocking solution - 5% nonfat dry milk in Blotting buffer.
Antibody solution - 5% nonfat dry milk in Blotting buffer.
Immunohistochemistry (Paraffin): 25ug/ml; Stains paraffin-embedded normal human liver sections.
Optimal dilutions to be determined by the researcher
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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