Produced in goats immunized with human alpha-1-Acid Glycoprotein (h alpha 1-Acid Glycoprotein) purified from human plasma. Human alpha 1-Acid Glycoprotein specific IgG was purified by human alpha 1-Acid Glycoprotein affinity chromatography. alpha 1-acid Glycoprotein, also known as orosomucoid-1, is a positive acute-phase reactant that is produced by the liver and secreted in plasma. Plasma alpha 1-acid concentration increases in response to inflammation and infection. alpha 1-acid glycoprotein belongs to the lipocalin family and binds
numerous basic and neutral lipophilic drugs and steroid hormones. It is a 183aa protein with 5 N-linked glycans that comprises 45% of the 43kD mass of the protein. The human and mouse protein share 43% aa sequence identity.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 0.5-1.0ug/ml
Western Blot: 0.1-0.2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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