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A33 (GPA33)

Cat no: A0000-33


Supplier: United States Biological
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Human A33, also known as GPA33, is a 43kD type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM A-C. The extracellular domain (ECD) of human A33 is 214 amino acids (aa) in length and contains one V-type and one C2-type Ig-like domain. Applications: Suitable for use in Flow Cytometry, Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 50ug/ml. 10ul labels 1-2.5x10e5 HT-29 cells in a reaction volume not exceeding 200ul. Direct ELISA: 0.5-1.0ug/ml; the detection limit is ~0.2ng/well. Western Blot: 0.1-0.2ug/ml; The detection limit is ~2ng/lane under non-reducing and reducing conditions. Immunohistochemistry: 2-15ug/ml detects A33 in cells and tissues. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A0000-33
Reactivities: Human
Hosts: Goat
Applications: ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
P type: Pab
Isotype: IgG
Purity: Purified by affinity chromatography
Additional info: Recognizes human A33. A33 is likely to be involved in cell-cell adhesion between epithelial cells. Crossreacts with recombinant mouse A33 ~10%.

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