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A4GNT (Alpha-1,4-N-acetylglucosaminyltransferase, Alpha4GnT, MGC149493)

A4GNT (Alpha-1,4-N-acetylglucosaminyltransferase, Alpha4GnT, MGC149493)

Cat no: A0001-35

Supplier: United States Biological
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This gene encodes a protein from the glycosyltransferase 32 family. The enzyme catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. It forms a unique glycan, GlcNAcalpha1-->4Galbeta-->R and is largely associated with the Golgi apparatus membrane. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Peptide ELISA Titer: 1:8000 Western Blot: 1-3ug/ml ~40kD band observed in lysates of cell line K562 (calculated MW of 39.5kD according to NP_057245.1) Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A0001-35
Reactivities: Human
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by peptide affinity chromatography.
Alternative names: EC=2.4.1.-
References: 1. Ishizone S, Yamauchi K, Kawa S, Suzuki T, Shimizu F, Harada O, Sugiyama A, Miyagawa S, Fukuda M, Nakayama J, Clinical utility of quantitative RT-PCR targeted to alpha1,4-N-acetylglucosaminyltransferase mRNA for detection of pancreatic cancer. Cancer science 2006 Feb 97 (2): 119-26.
Additional info: Recognizes human A4GNT. Species sequence homology: canine, bovine.

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