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ACAT1 (Acetoacetyl Coenzyme A thiolase, THIL, T2, MAT, ACAT, acetyl-Coenzyme A acetyltransferase 1, ACAT1)

Cat no: A0220-02


Supplier: United States Biological
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This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Peptide ELISA Titer: 1:2000 Western Blot: 0.1-0.3ug/ml ~45kD band observed in Human, Mouse and Rat Liver lysates (calculated MW of 45.2kD according to Human NP_000010.1 and 44.81kD to Mouse NP_659033.1). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A0220-02
Reactivities: Human, Mouse, Rat
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by peptide affinity chromatography.
Alternative names: acetoacetyl Coenzyme A thiolase, THIL, T2, MAT, ACAT, acetyl-Coenzyme A acetyltransferase 1, ACAT1
References: 1. Hongo S, Watanabe T, Arita S, Kanome T, Kageyama H, Shioda S, Miyazaki A, Leptin modulates ACAT1 expression and cholesterol efflux from human macrophages. American journal of physiology. Endocrinology and metabolism 2009 Aug 297 (2): E474-82.
Additional info: Recognizes human ACAT1. Species crossreactivity: mouse, rat. Species sequence homology: canine, bovine.

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