Acetate is an important component for biosynthesis. In living organisms, acetylation/deacetylation of proteins is critical for post-translational regulation of their functions. When bound to CoA, Acetyl-CoA is central to metabolism of carbohydrates and fats. It is also involved in the synthesis of biogenic compounds such as neurotransmitter acetylcholine.
Sample Type:
Serum, plasma, animal tissues (liver, kidney, muscle, heart etc), cell culture (adherent or suspension cells) and food.
Intended Use:
The kit can detect less than 20uM of acetate in various samples. Used for measurement of acetate in various tissues/cells and analysis of metabolism and cell signaling in various cells.
Test Principle:
In the Acetate BioAssay(TM) Kit, acetate is converted to an intermediate in the presence of Acetate Enzyme Mix & Acetate Substrate Mix. The intermediate reduces a colorless probe to a colored product with strong absorbance at 450nm. The Acetate Colorimetric Assay Kit is rapid, simple & sensitive. It can detect less than 20uM acetate in various samples.
Kit Components:
Acetate Assay Buffer, 1x27ml
Acetate Enzyme Mix, lyophilized, Ix vial
ATP, lyophilized, 1x vial
Acetate Substrate Mix, lyophilized, 1x vial
Probe, lyophilized, 1x vial
Acetate Standard, lyophilized, 1x vial
Storage and Stability:
Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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