Neuronal acetylcholine receptor is composed of alpha and beta subunits. The Alpha-4 subunit can be combined to beta-2 or beta-4 to give rise to functional receptors. Upon binding of acetylcholine, the acetylcholine receptor responds by undergoing an extensive change of conformation affecting all its subunits and lead to the opening of an ion-conducting channel across the plasma membrane. Neuronal acetylcholine receptor subunit alpha-4 is a transmembrane protein that localises to the cell junction, synapse and post synaptic cell membrane. It belongs to the ligand-gated ionic channel family. Abnormalities in the CHRNA4 gene cause autosomal dominant nocturnal frontal lobe epilepsy type 1. This disease is characterised by clusters of motor seizures during sleep.
Applications:
Suitable for use in ELISA, Western Blot. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20 degrees C. Aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Quick Search
Products 
