Home  >  Products  >  ACK1, phosphorylated (Tyr857/858) (Activated CDC42 Kinase 1, ACK-1, ACK, FLJ44758, FLJ45547, p21cdc42Hs, Tyrosine Kinase Non-receptor Protein 2, TNK2)
ACK1, phosphorylated (Tyr857/858) (Activated CDC42 Kinase 1, ACK-1, ACK, FLJ44758, FLJ45547, p21cdc42Hs, Tyrosine Kinase Non-receptor Protein 2, TNK2)

ACK1, phosphorylated (Tyr857/858) (Activated CDC42 Kinase 1, ACK-1, ACK, FLJ44758, FLJ45547, p21cdc42Hs, Tyrosine Kinase Non-receptor Protein 2, TNK2)

Cat no: A0650-01R

Supplier: United States Biological
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.Ack1 and Ack2 (activated cdc42-associated kinase 1 and 2) are non-receptor tyrosine kinases that consist of a tyrosine kinase core, an SH3 domain, a cdc42/Rac-binding (CRIB) domain, a Ralt homology region and a proline-rich region (1,2). Ack1 and 2 are the only two tyrosine kinases known to interact with cdc42. Both Acks are activated by growth factors including EGF and PDGF, as well as by activated integrins through cell adhesion, and may serve to link receptor tyrosine kinase or G protein-coupled receptor signaling with cdc42. Acks may regulate cell growth, morphology and motility (3,4). Recent findings indicate that Ack1 may play a role in prostate tumorigenesis, making it a potential drug target for this type of cancer (5). Phosphorylation of Ack1 on Tyr857 and Tyr858 on Ack1 was identified using LC-MS/MS platform for phosphorylation site discovery as well as other publications using MS technology (5,6). Phosphorylation of Ack1 at Tyr857/858 was observed in select carcinoma cell lines and in tumors. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A0650-01R
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by Protein A and peptide affinity chromatography.
Alternative names: EC=2.7.10.2, Tyrosine Kinase, Non-receptor, 2
Additional info: Recognizes overexpressed levels of Ack1 only when phosphorylated at Tyr857/858, transfected cells. Species Crossreactivity: mouse.

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