FHL proteins are tissue specific cofactors of CREB and CREM and participate in regulation of various physiological processes. These proteins are characterized by the presence of 4 LIM domains and a LIM-half domain at the N-terminus. ACT, a 33kD protein, is a novel member of this family. It is specifically expressed in male testis and consists of a central activation domain. It is coordinately expressed with CREM and plays a vital role in spermatogenesis. It binds specifically to CREM and powerfully stimulates its transcriptional activation, essential for differentiation of spermatids into mature spermatozoa. It activates CREM in a phosphorylation- and CBP-independent manner. Protein-protein interactions with a Kinesin KIF17B regulate ACT activity in mouse.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.