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Actin (ACTA2, ACTSA, actin, alpha 2, smooth muscle, aorta, alpha-cardiac actin)

Actin (ACTA2, ACTSA, actin, alpha 2, smooth muscle, aorta, alpha-cardiac actin)

Cat no: A0760-04B

Supplier: United States Biological
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Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. Applications: Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested. Recommended Dilution: Peptide ELISA: 1:4000 Western Blot: 0.1-0.3ug/ml. A band of ~45kD observed in human Duodenum lysates. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A0760-04B
Reactivities: Human, Mouse, Rat, Canine
Hosts: Goat
Applications: ELISA, Immunofluorescence, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris-saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Reddy S, Ozgur K, Lu M, Chang W, Mohan SR, Kumar CC, Ruley HE. Structure of the human smooth muscle alpha-actin gene. Analysis of a cDNA and 5' upstream region. J Biol Chem. 1990 Jan 25;265(3):1683-7.
Additional info: Recognizes human Actin. Species Crossreactivity: Canine, mouse, rat.

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