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Actin-(alpha)3/(gamma)2 (E2) pAb

Actin-(alpha)3/(gamma)2 (E2) pAb

Cat no: BS1004

Supplier: Bioworld Technology, Inc.
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All eukaryotic cells express actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of actin are present in mammalian tissues and fall into three classes. (alpha) actin expression is limited to various types of muscle, whereas (beta) and (gamma) are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the actin cytoskeleton. Rho controls the assembly of actin stress fibers and focal adhesion, Rac regulates actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.
Catalogue number: BS1004
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunofluorescence, Immunohistochemistry, Western Blot
Size: 100ug/100ul
Swiss prot: P63267
Dilutions: WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Form: liquid
Storage buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Concentration: 1ug/ul
Antigen: Synthetic peptide, corresponding to the N-terminal of Human Actin-(alpha)3/(gamma)2.
Species: Actin-(alpha)3/(gamma)2 (E2) pAb detects endogenous levels of Actin-(alpha)3/(gamma)2 protein.
Molecular weight: ~ 45 kDa
Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage temp: Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.
Alternative names: ACTA3; ACTL3; ACTSG; ACTG2; Alpha-actin-3; Gamma-2-actin; Smooth muscle gamma-actin; Actin; gamma-enteric smooth muscle
Additional info: For research use only, not for use in diagnostic procedure.

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