Home  >  Products  >  Actin, alpha cardiac muscle 1 (Alpha-cardiac actin, ACTC, Actin, alpha cardiac muscle 1, Alpha-cardiac actin, ASD5, CMD1R, CMH11)
Actin, alpha cardiac muscle 1 (Alpha-cardiac actin, ACTC, Actin, alpha cardiac muscle 1, Alpha-cardiac actin, ASD5, CMD1R, CMH11)

Actin, alpha cardiac muscle 1 (Alpha-cardiac actin, ACTC, Actin, alpha cardiac muscle 1, Alpha-cardiac actin, ASD5, CMD1R, CMH11)

Cat no: A0760-04C

Supplier: United States Biological
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Actin is an abundant cytoskeletal protein found in all cells (True, 1990). The protein's 42kD peptide chain assumes two physical forms: globular actin, which may serve as a cytoplasmic storage pool, and fibrous actin, which, in conjunction with myosin, generates muscle contraction (True, 1990). In non-muscle cells, actin appears to be involved in a variety of functions, such as cell motility, exocytosis, and phagocytosis (True, 1990). Distribution of the six known isoforms of actin-four muscle actins (alpha-skeletal, alpha-vascular smooth, alpha-cardiac, and gamma-enteric smooth) and two cytoplasmic actins (alpha and gamma) is tissue specific. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry, and Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:20 Western Blot: 1:1000 Immunohistochemistry (paraffin): 1:100-200 Immunocytochemistry: 1:250-500 Immunoprecipitation: 1:20 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A0760-04C
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
P type: Mab
Isotype: IgG
Purity: Supernatant
Additional info: Recognizes all human muscle Actin. Species Crossreactivity: mouse, rat.

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