Actin is a highly conserved protein which participates in a wide variety of cellular functions in eucaryotes including muscle contraction, amoeboid movement, cytokinesis, and mitotic division. Humans have six actin genes; two striated muscle types or sarcomeric (alpha-skeletal and alpha-cardiac), two smooth muscle types and two cytoskeletal forms (beta and gamma-types). The cytoplasmic beta and gamma actins are coexpressed in mammalian non-muscle cells, while the two smooth muscle actins are coexpressed only in smooth muscle with their relative amounts varying in different smooth muscle types. Similarly the sarcomeric actins, alpha-skeletal actin and alpha-cardiac actin are expressed in adult skeletal muscle and adult heart muscle respectively. Sarcomeric actins are major components of the thin filaments of striated muscle and it is the regulated interactions of actins by tropomyosin and the troponin complex with myosins of the thick filament which are responsible for contraction and generation of force. The two sarcomeric actin isoforms are encoded by different genes and are highly conserved. These two isoactins differ markedly in the 5' and 3' untranslated regions of the RNA and by fouraa at the protein level. The amount of the isoforms and the respective mRNA present varies with species, muscle type, development and aging.
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (formalin fixed paraffin embedded): 1:25-1:50
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.
Positive Control:
Skeletal muscle
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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