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Actin, alpha, Smooth Muscle

Cat no: A0760-27E


Supplier: United States Biological
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Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. Three main groups of actin isoforms have been identified in vertebrates; alpha, beta and gamma. Alpha actins are found mainly in muscle tissues. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml Human smooth muscle actin has a predicted length of 377 residues and a MW of 42kD. Immunohistochemistry (formalin fixed and paraffin): 0.2-2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS, pH 7.4, to concentration of 0.1-1mg/ml.. Reconstituted product is stable for 12 months at -20 degrees C. Aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A0760-27E
Reactivities: Human, Mouse, Porcine, Rabbit
Hosts: Mouse
Applications: Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.02% sodium azide. Reconstitute in 1ml of PBS to concentration of 0.1-1mg/ml.
P type: Mab
Isotype: IgG
Purity: Purified by affinity chromatography.
Alternative names: ACTA2, ACTSA, ACTVS
Additional info: Reacts only with alpha smooth muscle actin and does not react with actin from striated muscle (alpha-sarcomeric) and myocardium (alpha-myocardial). It also does not react with beta and gamma actins. Species crossreactivity: Human, mouse, porcine, baboon, rabbit.

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