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Actin, alpha, Smooth Muscle

Actin, alpha, Smooth Muscle

Cat no: A0760-30A

Supplier: United States Biological
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Alpha Smooth muscle actin is found in smooth muscle cells such as those in blood vessel wall regions of the intestine and myoepithelial glands. This antibody may be useful as a myogenic marker or to identify smooth muscle differentiation. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:100-1:250 Immunohistochemistry: 1:25-1:50 for 1 hr at RT Optimal dilutions to be determined by the researcher. Positive Control: Western Blot: Smooth muscle. Immunohistochemistry: Bowel wall, with distinct labeling of the muscularis mucosae and the muscularis propria (cytoplasmic staining pattern) Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A0760-30A
Reactivities: Human, Rat
Hosts: Mouse
Applications: Immunohistochemistry, Western Blot
Size: 1ml
Form: Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.
P type: Mab
Isotype: IgG2a
Purity: Supernatant
References: 1. Mason D and Gatter K. The role of immunocytochemistry in diagnostic pathology. Journal of Clinical Pathology. 40:1042-1054 (1987). 2. Skalli O, Ropraz P, Trzeciak A, et al.. A monoclonal antibody against a-smooth muscle actin: a new probe for smooth muscle differentiation. The Journal of Cell Biology. 103:2787-2796 (1986).
Additional info: Recognizes Smooth Muscle Actin, Alpha. Species Crossreactivity: Human and rat.

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