Home  >  Products  >  Actin, alpha, Smooth Muscle (Actin, Aortic Smooth Muscle, Alpha-actin-2, Cell Growth-inhibiting Gene 46 Protein, ACTA2, ACTSA, ACTVS, GIG46)
Actin, alpha, Smooth Muscle (Actin, Aortic Smooth Muscle, Alpha-actin-2, Cell Growth-inhibiting Gene 46 Protein, ACTA2, ACTSA, ACTVS, GIG46)

Actin, alpha, Smooth Muscle (Actin, Aortic Smooth Muscle, Alpha-actin-2, Cell Growth-inhibiting Gene 46 Protein, ACTA2, ACTSA, ACTVS, GIG46)

Cat no: A0760-26B

Supplier: United States Biological
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Ueyama et al. (1990) assigned the ACTSA gene to chromosome 10 by Southern blot analysis of DNAs from 18 rodent-human somatic cell hybrids. Regional mapping by in situ hybridization localized the gene to 10q22-q24. Assignment of the vascular smooth muscle actin gene ACTSA to human chromosome. Smooth muscle alpha-actin gene requires two E-boxes for proper expression in vivo and is a target of class I basic helix-loop-helix proteins. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 2ug/ml Immunohistochemistry (Formalin or acetone fixed): 0.3-1.2ug/ml Immunohistochemistry: Formalin fixed paraffin embedded Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with 1.2% sodium acetate or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A0760-26B
Reactivities: Human, Mouse, Rat
Hosts: Mouse
Applications: Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1ml 1.2% sodium acetate or PBS.
P type: Mab
Isotype: IgG2a
Purity: Purified by affinity chromatography.
References: 1. Kumar, MS. et al., (2003). Circ. Res. 92: 840-847. 2. Ueyama, H. et al. (1990). Jpn. J. Hum. Genet. 35: 145-150.
Additional info: Recognizes human Smooth Muscle Actin, alpha. Species Crossreactivity: mouse and rat

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