The rabbit anti-actin polyclonal antibody was raised against a synthetic peptide corresponding to N-terminus of human a-smooth muscle actin. This peptide sequence is identical in rat, mouse, chicken, bovine, etc. The other actin isoforms, such as a- and g-cytoplasmic, a-skeletal, a-cardiac actins, are not stained by this antibody.
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1ug/ml
Immunoprecipitation: 5-10ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.