Actins are highly conserved proteins which participate in a wide variety of cellular functions in eucaryotes including muscle contraction, amoeboid movement, cytokinesis, and mitotic division. Humans have six actin genes; two striated muscle types or sarcomeric (alpha-skeletal and alpha-cardiac), two smooth muscle types and two cytoskeletal forms (beta and gamma-types). The cytoplasmic beta and gamma actins are coexpressed in mammalian non-muscle cells, where it functions to control cell structure and motility. The two smooth muscle actins are expressed in smooth muscle and the sarcomeric actins, alpha-skeletal actin and alpha-cardiac actin are expressed in adult skeletal muscle and adult heart muscle respectively, regulating contractile potentials for these cells. It has been reported that actin is hyperphosphorylated in primary breast tumors. It has also been shown that actin dysfunction leads to certain types of heart failure.
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (paraffin): 1:25-1:50
Optimal dilutions to be determined by the researcher.
Positive Control:
Skeletal muscle
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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