Actin is a semi-flexible polymer found as a part of the cytoskeleton of cells in higher biological organisms. It is a highly conserved protein which participates in a wide variety of cellular functions in eucaryotes including muscle contraction, amoeboid movement, cytokinesis, and mitotic division. Actin protein as a polymer without attached proteins is called filamentous actin (F-actin), while the globular actin monomers are called G-actin. Humans have six actin genes; two striated muscle types or sarcomeric (alpha-skeletal and alpha-cardiac), two smooth muscle types ands two cytoskeletal forms (beta and gamma-types). Alpha-actin is found in muscle cells where it plays an important role in contracting the cell. Whereas beta-actin is localized in the front of moving cells and gamma-actin forms stress fibers. The cytoplasmic beta and gamma actins are coexpressed in mammalian non-muscle cells, while the two smooth muscle actins are coexpressed only in smooth muscle with their relative amounts varying in different smooth muscle types. Similarly the alpha-skeletal actin is thought to be the only actin expressed in adult skeletal muscle, and alpha-cardiac actin is thought to be the only actin expressed in adult heart muscle.
Applications:
Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 20ug/ml
Optimal dilutions to be determined by the researcher.
Positive Control: Muscle or sarcoma
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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