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Actin, pan (alpha, beta, gamma) (FITC)

Actin, pan (alpha, beta, gamma) (FITC)

Cat no: A0759-10D

Supplier: United States Biological
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Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis. Actin is one of the most conserved cellular protein. At least 6 actin isoforms have been identified by protein sequence analyses. Four actin isoforms represent the differentiation markers of muscle tissues. There are three a-actins: a-skeletal, a-cardiac, and a-smooth muscle), one b-actin (b-non-muscle), and two g-actins (g-smooth muscle and g-non-muscle). Actin isoform are ~90% conserved, except in the N-terminal 18aa (50-60%homology). Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western or Northern blots. Applications: Suitable for use in Immunofluorescence. Other applications not tested. Recommended Dilutions: Immunofluorescence: 1:50-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Catalogue number: A0759-10D
Reactivities: Human, Mouse, Rat, Bovine, Canine, Chicken/Bird, Goat, Guinea Pig, Porcine, Rabbit, Sheep
Hosts: Mouse
Applications: Immunofluorescence
Size: 100ul
Form: Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide. Labeled with Fluorescein isothiocyanate (FITC)
P type: Mab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Ohmuri H (1995) Gene Accession # S38782. 2. Vandekerchove, J et al (1978) Eur. J. Biochem. 90, 451. 3. Lessard J et al (1988) Cell. Motil Cytoskel. 10, 349. 4. North JA et al (1994) J. Cell Sci. 107, 437.
Additional info: Recognizes human Actin. Species Crossreactivity: bovine, sheep, goat, porcine, rabbit, canine, mouse, rat, guinea pig, hamster, chicken, carp, frog and snail.

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