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Actin, Smooth Muscle (alpha-Actin 2, ACTA2)

Actin, Smooth Muscle (alpha-Actin 2, ACTA2)

Cat no: A0759-05D

Supplier: United States Biological
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Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. In vertebrates three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunofluorescence: 1:25-1:50 (cells) and 1:100 (vessel sections) Immunohistochemistry (FFPE or frozen): 1:50-1:75. Incubate for 30 minutes at RT. Immunocytochemistry: 1:75 Optimal dilutions to be determined by the researcher. Positive Control: Leiomyoma Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A0759-05D
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Size: 500ul
Form: Supplied as a liquid in 20mM Tris-borate, 150mM sodium chloride, 0.09% sodium azide.
P type: Mab
Isotype: IgG2a,k
Purity: Supernatant
Alternative names: ACTSA, ACTVS, ACTSA, alpha Smooth Muscle Actin
References: 1. Skalli O; Alpha-smooth muscle actin, a differentiation marker of smooth muscle cells, is present in microfilamentous bundles of pericytes. J Histochem Cytochem:315-21 (1989). <PUBMED:2918221; 2. Skalli O;; Myofibroblasts from diverse pathologic settings are heterogeneous in their content of actin isoforms and intermediate filament proteins; Lab Invest. 60:275-85 (1989). PUBMED:2644484; 3. Skalli O. et al.; A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation; J. Cell Biol. 103:2787-96 (1986). PUBMED:3539945
Additional info: Recognizes the alpha smooth muscle isoform of human actin, smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, and ovary. Does not react with actin from fribroblasts, striated muscle or myocardium.

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