ADP is a product of ATP dephosphorylation and it can be rephosphorylated to ATP. Dephosphorylation and rephosphorylation occur via various phosphorylases and kinases. ADP is stored in platelets and can be released to interact with variety of purinergic receptors. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast although several such processes occur in the cytoplasm. Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, the luciferase system is unstable and luminescence equipment is not generally available in most laboratories.
Sample Type:
Animal tissues: Liver, Muscle, Heart etc. Cell culture: Adherent or suspension cells
Intended Use:
ADP BioAssay(TM) kit II is suitable for measuring ADP levels in samples that contain reducing substances, which may interfere with oxidase-based assays. ?
Test Principle:
In this assay, ADP in the presence of ADP Enzyme Mix is converted to an intermediate, which reduces a colorless Probe to a colored product with strong absorbance at 450nm. ADP BioAssay(TM) Kit II is simple, fast and high-throughput ready. It can detect less than 20uM of ADP in samples.
Kit Components:
ADP Assay Buffer, 1x27ml
ADP Enzyme Mix, lyophilized, 1x vial
ADP Developer, lyophilized, 1x vial
Probe, lyophilized, 1x vial
ADP Standard, lyophilized, 1x vial
Storage and Stability:
Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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