HiTSeeker ADRA1A Assay Cell Line contains U2OS cells stably expressing human Alpha-1A adrenergic Receptor (ADRA1A) with no tag. Innoprot HiTSeeker ADRA1A Assay Cell Line has been designed to assay compounds or analyze their capability to modulate Alpha-1A adrenergic Receptor. When epinephrine binds to the ADRA1A a G protein is activated, which in turn, triggers a cellular response mediated by second messengers (e.g. Calcium). This cell line has been validated measuring calcium increase in the cytosol. The high reproducibility of this assay allows monitoring ADRA1A receptor activation process in High Throughput Screening.
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