Akt is a protein kinase that can be activated by insulin and various growth factors and functions in a pathway involving PI3 kinase. Recent evidence suggests that Akt functions to promote cell survival by actively inhibiting apoptosis. The Akt Activity Assay Kit utilizes an Akt-specific antibody to immunoprecipitate Akt from cell lysate. Activity of the Akt is then determined in a kinase reaction using recombinant GSK-3a as substrate. Phosphorylation of the GSK-3a can be analyzed by Western blot analysis using the phospho-GSK-3a specific antibody included in the kit. The kit specifically detects Akt1, Akt2, and Akt3 activities, other kinase activities would not be detected.
Sample type:
Cell and Tissue lysates
Intended Use:
The Akt Activity Assay Kit utilizes an Akt-specific antibody to immunoprecipitate Akt from cell lysate. Activity of the Akt is then determined in a kinase reaction using recombinant GSK-3a as substrate.
Specificity:
Mammalian
Kit Components:
Kinase extraction buffer, 1x80ml
Akt specific antibody, 1x80ul
Protein a sepharose, 1x2ml
GSK-3a protein/ATP mixture, 1x80ul
Kinase assay buffer, 1x25ml
Phospho-GSK-3a specific antibody, 1x50ul
Storage and Stability:
Store at -20 degrees C. Store other components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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