Arabinose Inducible Prokaryotic Dre Recombinase Expression Plasmid: pSC101-BAD-Dre (Requires limited use license)

Arabinose inducible prokaryotic Dre recombinase expression plasmid (Academia).The use of this product is governed by the terms and conditions of the limited use license (Dre Material). Industrial clients require a license from Stowers Institute for Medical Research, Kansas City, Missouri.Site-specific recombinases (SSRs) like Cre or Dre are valuable tools in functional genomics and have been applied in various organisms. They mediate recombination between target sites of 32-34 base pairs (bp) in length. The target sites, which are called loxP or rox sites are 13-14 bp palindromes separated by spacers (see below).Cre recombinase, which was originally isolated from coliphage P1, mediates recombination between two loxP-sites through the spacer regions (e.g. removal of selectable genes). Dre was identified in a systematic search through P1-like phages for a Cre-like enzyme that had diverged sufficiently to recognize a recombination target site ( RT) that is distinct from loxP (Sauer and Mc Dermott, 2004).The combination of the arabinose inducible BAD promoter and the low-copy pSC101 plasmid backbone provide an excellent on-off regulation of Cre or Dre in E. coli as proved in a test experiment (Anastassiadis et al. 2009).The plasmids carry a tetracyclin resistance gene for selection and are compatible with plasmids based on a ColE1 or p15A origin of replication and an ampicillin or kanamycin resistance marker.While Cre/loxP is widely used in mouse genetics for conditional mutagenesis with many mouse lines available, a second highly efficient system like Dre/rox opens the door for more complex tasks such as a conditional mutagenesis of alternatively spliced exons. Cre/loxP can be used to remove one alternative exon and Dre/rox to remove the other one.ContentsRecombinase expression plasmid pSC101-BAD-Dre (0.2 ug/ul, 20 ul)Manual