BCA Protein BioAssay(TM) Kit II is used for measuring total protein concentration of pure proteins, extracts or lysates. The kit also includes Bovine Serum Albumin (BSA) as a protein standard for estimation of total protein content of samples.
Test Principle:
BCA Protein BioAssay(TM) kit provides a colorimetric detection and quantification of total protein content even in the presence of detergents. The kit is based on the chelation of bicinchoninic acid (BCA) with the cuprous cation (Cu+1), which is generated by reduction of cupric cation (Cu+2) with the protein in an alkaline condition. The Cu+1-BCA chelate is a water-soluble complex and exhibits a strong absorbance at 562nm that is linear over a wide range of protein concentrations between 25-2000ug/ml. In general, protein concentrations are estimated with reference to a commonly used protein standard.
Kit Components:
BCA Reagent A, 1x500ml
BCA Reagent B, 1x25ml
BSA Standard (2mg/ml), 5x1ml
Storage and Stability:
Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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