Human bIG-H3 (also known as Kerato-epithelin, RGDCAP) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human bIG-H3 in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human bIG-H3 coated on a 96-well plate. Standards and samples are pipetted into the wells and bIG-H3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human bIG-H3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of bIG-H3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Sample Type:
Serum, Plasma, Cell culture supernatants, Urine
Intended Use:
quantitative measurement of human bIG-H3 in serum, plasma, cell culture supernatants and urine.
Sensitivity:
Reproducability CV is <12%. Intra assay CV is <10%.
Specificity:
Recognizes human bIG-H3.
Kit Components:
bIG-H3 microplate (96 wells) coated with anti-human bIG-H3, 1
Wash bufer concentrate (20x), 1x25ml
Standards (human bIG-H3), 2 vials.
Assay diluent buffer A (0.09% sodium azide), 1x30ml
Assay diluent buffer B (5x), 1x15ml
Detection antibody bIG-H3, 2 vials.
HRP-streptavidin concentrate (300x), 1x200ul
TMB one step substrate reagent, 1x12ml
Stop solution (0.2M sulfuric acid), 1x8ml
Storage and Stability:
Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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