BIO-X-ACT(TM) Short DNA Polymerase is a high-performance enzyme designed for difficult/problematic PCR applications requiring high processivity and fidelity. Two versions of BIO-X-ACT are available, for amplification of short and long DNA fragments respectively, and are the polymerases of choice for difficult applications that would normally fail with standard Taq polymerases. BIO-X-ACT Short is recommended for short genomic DNA fragments of up to 2Kb, or Lambda DNA fragments up to 5Kb. Using Lambda DNA as template, the best performance is achieved within the 100bp-3Kb range. For longer DNA fragments, see BIO-X-ACT(TM) Long DNA Polymerase. For enhanced specificity, BIO-X-ACT Short is supplied with a vial of 5x Hi-Spec Additive. Hi-Spec Additive is a very efficient enhancer, which helps to prevent the formation of false background bands and smearing. The specificity and performance can be further improved with the use of 4x PolyMate Additive (not supplied, see associated products), which is designed for GC or AT-rich DNA, \dirty\" templates or sequences with difficult melting profiles."
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