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Biotin (B-Galactosidase)

Cat no: 168054


Supplier: United States Biological
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Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptavidin; an attraction that is the strongest and most stable non-covalent interaction known. B-galactosidase (b-gal; b-gal) is an enzyme that hydrolyzes b-galactosides (such as lactose and various glycoproteins) into simple sugars. B-galactosidase is routinely used as a reporter in biochemical experiments as well as a marker for gene expression experiments in E. coli. B-galactosidase can be used to cleave the chemical X-gal that forms an intense blue product that can easily be quantified. Source: B-Galactosidase, expressed in E.coli. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:8000-1:32,000 Western Blot: 1:500-1:2500 Immunohistochemistry: 1:200-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C. For long-term storage, aliquot and store at 4 degrees C. Do not freeze. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: 168054
Applications: ELISA, Immunohistochemistry, Western Blot
Size: 1mg
Form: Supplied as a liquid in 0.05M potassium phosphate, 0.001M magnesium chloride, 0.1M beta-mercaptoethanol, pH 7.8. Conjugated to beta-Galactosidase.
Purity: Purified

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