BMP-2, Human, BioAssay(TM) ELISA Kit

Human BMP-2 (Bone morphogenetic proteins-2) ELISA (Enzyme-Linked Immunosorbent Assay) BioAssay(TM) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BMP-2. This assay employs an antibody specific for human BMP-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human BMP-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm. The minimum detectable dose of BMP-2 is typically less than 45 pg/ml. Source: Human Sample Type: Serum, plasma, cell culture supernatants and urine. Intended Use: This ELISA BioAssay(TM) kit is specific for the measurement of human BMP-2. In this ELISA format, the pair antibody shows less than 1% cross-reactivity with rhBMP-3, rhBMP-4, rhBMP-5, rhBMP-6 or rhBMP-7. Sensitivity: Reproducability CV is <10% and inter-Assay is CV <12%. Kit Components: BMP-2 Microplate (Item A) coated with anti-human BMP-2, 1x96wells Wash Buffer Concentrate (Item B), 1x25ml Standards (Item C), 2x vials Assay Diluent A (Item D), 1x30ml Assay Diluent B (Item E), 1x15ml Detection Antibody BMP-2 (Item F), 2x vials HRP-Streptavidin (Item G),1x200ul TMB One-Step Substrate Reagent (Item H), 1x12ml Stop Solution (Item I), 1x8ml Storage and Stability: Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.