BMP-6, Human, BioAssay(TM) ELISA Kit

Human BMP-6 (Bone morphogenetic proteins-6) ELISA (Enzyme-Linked Immunosorbent Assay) BioAssay(TM) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BMP-6. This assay employs an antibody specific for human BMP-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human BMP-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm. The minimum detectable dose of BMP-6 is typically less than 150 pg/ml. Source: Human Sample Type: Serum, plasma, cell culture supernatants and urine. Intended Use: The BioAssay(TM) kit is used for quantitative protein detection, establishing normal range and validation of antibody array results. The antibody pair provided in this kit recognizes human BMP-6. Sensitivity: The intra-Assay reproducibility is CV<10% and inter-Assay is CV<12%. Kit Components: BMP-6 Microplate (Item A), 1x96wells Wash Buffer Concentrate, 1x25ml Standards (Item C), 2x vials Assay Diluent (Item E), 15ml Detection Antibody Angiostatin (Item F), 2x vials HRP-Streptavidin Concentrate (Item G), 1x200ul TMB One-Step Substrate Reagent (Item H), 1x12ml Stop Solution (Item I), 1x8ml Storage and Stability: Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.