BMPs (bone morphogenetic proteins) belong to the TGF-b superfamily of structurally related signaling proteins. As implied by their name, BMPs promote and regulate bone development, growth, remodeling and repair, in both prenatal development and postnatal growth of eye, heart, kidney, skin, and other tissues. Recombinant human BMP-7 produced from E. coli is a 16kDa monomeric, non-glycosylated, polypeptide chain containing 139aa residues.
Source:
Recombinant corresponding to human BMP-7 protein expressed in E.coli.
Molecular Weight:
~16kD
Biological Activity:
Recombinant human BMP-7 is biologically active. The ED50 as determined by dose-dependent induction of alkaline phosphatase activity with C2C12 cells is in the range of 2-12ug/ml.
Endotoxin:
~0.1ng/ug
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer