c-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins, and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. The MAP kinase homologue, JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. The activity of JNK is stimulated by the same signals that activates c-Jun.
Source:
c-Jun fusion protein was expressed in E. coli. It is expressed as a recombinant protein fusion of human c-Jun residue 1-79 and glutathione S-transferase.
MOL. WEIGHT: 37kD
ACTIVITY:
C-Jun fusion protein at a concentration of 2ug/20ul reaction was phosphorylated in an in vitro kinase assay with 200uM ATP. After 30 minutes incubation at 30 degrees C, phosphorylation was detected by Western blot with phospho-specific c-Jun antibodies.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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