Cathepsin G is an enzymatic protein belonging to the peptidase or protease families. The protein is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation.
Sample Type:
Cell and tissue lysates, serum
Intended Use:
Detects alternative proteolytic enzymes such as the lysosomal cathepsin G proteases that can initiate or propagate proapoptotic signals.
Test Principle:
In the Cathepasin G Activity BioAssay(TM) Kit, Cathepsin G will cleave the substrate and release the dye group, pNA (4-Nitroaniline), which can be detected at 405nm. In presence of the Cathepsin G specific inhibitor, the cleavage will be stopped. The kit provides a rapid, simple, sensitive, and reliable test suitable for the activity of Cathepsin G.
Kit Components:
Assay Buffer, 1x25ml
Substrate, 1x200ul
Cathepsin G Inhibitor, 1x20ul
pNA Standard, 0.1M, 1x20ul
Positive Control, 1x vial
Storage and Stability:
Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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