The Mammalian Cell Extraction BioAssay(TM) Kit provides optimized cell extraction buffer, protease inhibitor cocktail, and DTT for convenient extraction of mammalian proteins from cultured cells and tissue samples, under nondenaturing conditions.
Sample Type:
Cell culture (adherent and suspension), fresh and frozen tissues.
Intended Use:
Cell lysate prepared using the kit can be used in a variety of applications, such as enzyme activity assays (e.g., caspase activity assays), Western blot analysis, and others. The entire procedure takes less than 20 minutes.
Kit Components:
Extraction Buffer, 1x50ml
Protease Inhibitor Cocktail, 1x vial
DTT, 1M, 1x110ul
Storage and Stability:
Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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