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Cellulase (Onozuka R-10)

Cellulase (Onozuka R-10)

Cat no: C2610-11

Supplier: United States Biological
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Cellulase (Onozuka R-10) is a multi-component enzyme system derived from Trichoderma viride and contains cellulase, alpha-amylase, hemicellulase, pectinase and protease activities. It is often used in the isolation of plant protoplasts in combination with other enzymes. Unit Definition of Activity: One unit of Cellulase will liberate 1.0 micromole of glucose form carboxymethyl cellulose per minute at 40 degrees C, pH 4.5; glucose determined with alkaline copper reagent (3). Major Enzyme Activity: Cellulase: 1.0 U/mg Extraneous Enzyme Activities: Pectinase: 0.4U/mg Hemicellulase: 1.0U/mg alpha-Amylase: 0.6U/mg Protease: 0.01U/mg Optimum pH range: 4-5 Solubility: Soluble in water (10mg/ml) Stability and Storage: Lyophilized powder may be stored at 4 degrees C. Stable for 12 months at 4 degrees C.
Catalogue number: C2610-11
Size: 1g
Form: Supplied as a lyophilized powder.
References: 1. Beldman, G. et al. (1985) Eur. J. Biochem. 146, 301-8 2. Potrykus, J. & Shillito, R.D. (1986) Methods Enzymol. 118, 549-78 3. Okada, G. (1988) Methods Enzymol. Vol. 160, 259-63 4. Lendl & Bauer (1989) Zell- und Gewebekultur, Gustav Fischer Verlag, 147ff.

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