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Chorionic Gonadotropin, Human, beta (hCGb)

Chorionic Gonadotropin, Human, beta (hCGb)

Cat no: C5069-86

Supplier: United States Biological
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Human Chorionic Gonadotropin (HCG) is a glycoprotein, which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera and rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the a-and b-subunits. The a-subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP), and HCG but negative for EMA and AFP is probably a choriocarcinoma. Application: Suitable for antigenic applications in immunological protocols. Recommended Dilutions: Optimal dilutions to be determined by researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Powder is stable for 12 months at -20 degrees C. Aliquot reconstituted product and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C5069-86
Applications: ELISA
Size: 100ug
Form: Supplied as a lyophilized powder from 50mM ammonium bicarbonate. Reconstitute with PBS, pH 7.5, at 1-2mg/ml.
Purity: (same/more than)98% by SDS-PAGE. Purified by HPLC. Contaminants: hCG: 1.4%; LH: <0.0001%; FSH: <0.0001%; TSH: <0.0001%
References: US Biological application reference: He, L.-Z. et al., (2004) Clinical Cancer Research 10:1920.

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