Protease inhibitor produced by Actinomycetes. Inhibits serine and cysteine proteases: a, b, g, d chymotrypsin, papain, cathepsin A, B and C. Mixture of Types A, B and C where A=Leu, B= Val, C= Ile. Mostly Type A.
Solubility (10mg/ml, glacial acetic acid): Colorless to yellow, clear, complete
Loss on Drying: <3%
Percentage of X
components:
Leu: ~75%
Val: ~20%
Ile:~5%
Activity (IC50) (alpha-chymotrypsin/casein, pH 7.4, 37 degrees C): ~0.2ug/ml
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile glacial acetic acid. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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