Citrulline exists primarily in the liver, where it is involved in the urea cycle to detoxify and excrete ammonia. This unusual amino acid is formed in the urea cycle by the addition of carbon dioxide and ammonia to ornithine. It is combined with aspartic acid to form arginosuccinic acid, which later is metabolized into the amino acid arginine. In bacteria, the arginine dihydrolase pathway deaminates arginine to citrulline and splits citrulline into ornithine and carbamoyl phosphate. This pathway is a source of energy for many microorganisms and is used as an identifying test. Citrulline is not a component of any major proteins or enzymes.
Applications:
Suitable for use in ELISA, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, 0.1% merthiolate. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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