Clostridium difficile Toxoid B is a highly purified, non-toxic antigen preparation derived from Clostridium difficile Toxin B. It has been developed either for use as an immunogen to raise antibodies or for use in clinical diagnostic assays. Formaldehyde is use to convert toxin to toxoid and quality control data illustrates that this treatment has no significant effect on the antigenicity of the resultant preparation.
Source:
Native toxin B, starin VPI10463, from C. difficile, converted to inactive toxoid form.
Applications:
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Inactivation:
Formaldehyde. No cell rounding in the sensitive verocytotoxicity assay at 2ug/ml compared to active toxin lethal dose of 4pg/ml.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Note:
No test guarantees a product to be non-infectious. All materials should be handled as if potentially infectious. Generally accepted laboratory practices appropriate for infectious materials should be employed when handling this product.
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