Clostripain (Endoproteinase Arg C)

Cat no: C5860

Supplier: United States Biological

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Clostripain (Endoproteinase-Arg-C) is a two chain proteinase associated with collagenase and isolated from Clostridium histolyticum. It is highly specific for the carboxyl peptide bond of arginine. Clostripain has a sulfhydryl requirement; it is activated by dithiothreitol, cysteine, or other sulfhydryl containing reagents. The presence of calcium ions is essential. The enzyme is inhibited by oxidizing agents and sulfhydryl reactants and by Co2+, Cu2+, Cd2+, and heavy metal ions. Citrate, borate, and Tris anions are less inhibitory. Activity: ~50 units/mg dry weight Unit Definition: 1 unit hydrolyzes 1umole of N-benzoyl-L-arginine ethyl ester (BAEE) per minute at 25C, pH 7.6, in the presence of 2.5mM dithiothreitol Quality Control: SDS-PAGE Storage and Stability: Lyophilized powder may be stored at 4 degrees C. Stable for 9-12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Catalogue number:	C5860
Size:	5mg
Purity:	Supplied as a dialyzed, lyophilized powder.
Alternative names:	EC=3.4.22.8; Endoproteinase Arg C
References:	1. Gilles, A., et al., (1979) J. Biol. Chem., 254:1462. 2. Gilles, A.,et al., (1984) Eur. J. Biochem., 145:469. 3. Homandberg, G., et al., (1982) Biochem., 21:3385, 4. Kembhavi, A., et al., (1991) EBS Lett., 283:277. 5. Meiwes, J., et al., (1991) Biomed. Biochim. Acta, 50:S80. 6. Mitchell, W., (1968) Science, 162:374. 7. Mitchel, W., and Harrington, W. , (1968) J. Biol. Chem., 243:4683.

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Clostripain (Endoproteinase Arg C)

Cat no: C5860

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