Creatine Kinase Activity BioAssay(TM) Kit (Colorimetric)

Creatine Kinase (CK) also known as creatine phosphokinase (CPK) and ATP: creatine N-phosphotransferase is a common cellular enzyme (EC 2.7.3.2). It catalyzes the reversible conversion of creatine and ATP into ADP and phosphocreatine. CK is widely expressed in various tissues and cell types, with highest activity in striated muscles, heart tissue and brain. CK consists of two subunits: M (muscle) and B (brain), and has three isoenzymes: CK-MM (skeleton muscle), CK-MB (cardiac muscle), and CK-BB (brain). Increased CK level is associated with many diseases such as myocardial infarction, muscular dystrophy, pulmonary infarction and brain tumors. Accurate measurement of CK is crucial for early diagnosis, prediction and therapeutic strategy. Sample Type: Serum and plasma, animal tissues: muscle, brain, heart etc. and cell culture: adherent or suspension cells. Intended Use: Kit contains all necessary reagents for measuring creatine kinase activity and can detect creatine kinase activity less than 1mU. Test Principle: In the Creatine Kinase Activity Colorimetric BioAssay(TM) kit, creatine kinase converts creatine into phosphocreatine and ADP. The generated phoshocreatine and ADP reacts with CK Enzyme Mix to form an intermediate, which reduces a colorless Probe to a colored product with strong absorbance at 450nm. The CK Activity Assay is high-throughput adaptable, simple and sensitive. Kit Components: CK Assay Buffer, 1x25ml CK Substrate, 1x1.1ml ATP, Lyophilized, 1x vial CK Enzyme Mix, Lyophilized, 1x vial CK Developer, Lyophilized, 1x vial NADH Standard, Lyophilized, 1x vial Positive Control, Lyophilized, 1x vial Storage and Stability: Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.